Bucladesine

Gusaba
Dibutyryl cyclic adenosine monophosphate sodium ni inkomoko ya cyclic adenosine monophosphate, ikoreshwa mu kuvura ihungabana ry'umutima no kunanirwa k'umutima.Mu 1999, amavuta ya Dibutyryl cyclic adenosine monophosphate sodium yakozwe kugirango avure indwara zuruhu n ibisebe byuruhu.

Gutegura sodium dibutyryl cyclophosphate adenosine: Muri flask irimo cyclophosphate adenosine yongeramo buhoro buhoro igisubizo cyamazi ya hydroxide ya sodium (3 mol / L) gitonyanga, inzira yagenzuye ubushyuhe, ubushyuhe bwarangije gushyuha mubushyuhe bwicyumba, hindura pH kuri 7 ~ 8 hamwe nigisubizo cya HCl ( 20%), ongeramo imvura ya anhydrous ethanol yimvura yera, kuyungurura, kumisha kugirango ubone umunyu wa sodium yera ikomeye;umunyu wa sodium na n-butyl anhydride mukurinda ubushyuhe bwa N2 kugeza 140 ℃ reaction.Gukurikirana TLC, reaction iruzuye hanyuma ikamanurwa kubushyuhe bwicyumba, ongeramo buhoro buhoro methyl tert-butyl ether, inzira yo gukurura imbaraga, Igitabo cya Chemical kugirango ubone ibicuruzwa byera byera byera.Uruvange rwa methyl tert-butyl ether na acetone rwahagaritswe kugirango ubone ibicuruzwa byifu byifu.Igikorwa cyibinyabuzimaDibutyryl-cAMP (Bucladesine, dbcAMP) numusemburo wa PKA ukora utugingo ngengabuzima ukora wigana ama endogenous cAMP.Dibutyryl-cAMP (Bucladesine) nayo ni inhibitori ya fosifisiyose (PDE).
Mu bushakashatsi bwa vitro: Dibutyryl-cAMP ibuza gufata glucose ya neuronal ikoresheje PKA.Imbeba hepatocytes yimico, Dibutyryl-cAMP ibuza imvugo ya nitric oxyde idasobanutse hamwe nibikorwa bya NF-kappaB.dibutyrChemicalbookyl-cAMP irabuza kandi TNFalpha iterwa na selile selile apoptose mukubuza kugenzura FADD.
Mu bushakashatsi bwa vivo: Muburyo bwimbeba, Bucladesine (600nM / imbeba, ip) yahinduye zinc chloride na sisitemu ya acetate itera kwibagirwa kwibuka.